FGSC #4298

Reporting Genes: ure-1 his-1

Species: crassa

Allele: 9 C91

Alternate Strain Number: 600-75

Depositor: HGK

Linkage Group: VR VR

Mating Type: a

Species Number: 10

ref1: Buremalm & Kolmark 1971 Hereditas 69:249-262, https://doi.org/10.1111/j.1601-5223.1971.tb02440.x

Genes

Locus	Cultural Requirements	Link Group	Type
his-1	VR. Right of ure-1 (1%). Left of pho-2 (3%), al-3, and inl (1 to 10%) (397, 570, 578, 1036). (434)Requires histidine (434). Accumulates imidazole glycerol phosphate. Lacks imidazole glycerol phosphate dehydrase (24, 25) (Fig. 14). Intralocus complementation (162). Recombination between his-1 alleles is controlled by rec-1(172, 520, 1070). Initial his-1 allele called C84.	VR	B
ure-1	VR. Right of ace-5 (<1%), am (1%), and ure-2 (3%). Left of his-1 (<1%) (570, 578). No urease activity (452, 569) (Fig. 10 and 24). Some revertants show altered heat stability of urease, suggesting a structural role (78). Used to study the metabolic fate of arginine by measuring urea accumulation (592). Used for arginine tracer experiments (217); flux (355). Used to determine the relative contributions of arginine and purines in urea formation (235). For the role of urease in purine catabolism, see Fig. 24. For scoring methods, see ure. Possible aflele D2 fails to complement ure-1, -2, -3, or -4 (452). Called ure(9) (570).	VR	B

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