FGSC #4285

Reporting Genes: udk uc-4

Species: crassa

Allele: FB-D425 RW341

Alternate Strain Number: D425a

Mutagen: UV

Depositor: FPB

Linkage Group: VR VR

Mating Type: a

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 4284

ref1: Buxton Mol Gen Genet 185:129-131 1982, https://doi.org/10.1007/BF00333801

ref2: Buxton Mol Gen Genet 185:132-135 1982, https://doi.org/10.1007/BF00333802

Genes

Locus	Cultural Requirements	Link Group	Type
uc-4	VR. Between inl (12%) and his-6 (11%) (126). Not in I as reported in references 1139 and 1141 on the basis of one ascus in eight recombinant with mt. Thymidine salvage pathway defect (Fig. 23). Unable to use thymidine, thymine, 5-hydroxymethyluracil, 5-formyluracil, or uracil to support pyr-1, even in the presence of a uridine primer (1141). Deficient in phosphoribosyl transferase (127). The uc-4 single mutant converts 50% of the supplied uridine to uracil and excretes it into the medium (1141). Resistant to 5-fluorouracil (126).	VR	B
udk	VR. Left of uc-4 (29%) (126). Deficient in uridine kinase (127) (Fig. 23). The udk uc-4 double mutant is resistant to 5-fluoro-deoxyuridine and 5-fluorouridine (126), but the udk single mutant is not resistant to any analog. Uracil salvage pathway, by which many exogenously supplied pyrimidines are cycled via uracil to uridine 5'-phosphate and, thus, into the latter part of the pyrimidine biosynthetic pathway. The figure shows the sites of action of the salvage pathway uc genes, the ud and uc-5 genes, which control uptake of exogenous uridine and uracil, and udk, which controls uridine kinase (126, 420, 616, 977, 1141). For endogenous pyrimidine synthesis, see Fig. 20.	VR	B

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