FGSC #4270

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Reporting Genes: leu-1

Species: crassa

Allele: D221

Alternate Strain Number: D221-2-19c

Depositor: ELT

Linkage Group: IIIR

Mating Type: a

Species Number: 10

ref1: Gross PNAS 54(6)1538-1546, https://doi.org/10.1073/pnas.54.6.1538

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B