FGSC #4140

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Reporting Genes: rib-1 pan-2 trp-2

Species: crassa

Allele: 51602(t) B2(Y153M66) DRS41

Alternate Strain Number: M1805

Depositor: EK

Linkage Group: VIC VIR

Mating Type: a

Species Number: 10

Opposite Mating Type: 4139

ref1: Kafer 1982. Neurospora Newsl. 29:41-44, https://doi.org/10.4148/1941-4765.1645

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B
rib-1	VIR. Between T(AR209) and pan-2 (3%). Right of ad-1 (3 to 6%), the centromere (1%), and glp-4 (4%) (486, 1012, 1102). (482) Requires riboflavin (681). Used to demonstrate role of flavin as a photoreceptor for carotenogenesis and for phase shifting and suppression of circadian conidiation (775). Allele 51602 is heat sensitive (34°C versus 25 C); allele C106 is not (380).	VIR	B
trp-2	VIR. Right of del (0 to 13%). Left of un-23 (5 to 27%), T(OY320), and ws-1 (38%) (818, 822, 1019, PB). Uses kynurenine, anthranilic acid, indole, or tryptophan (96). Kynurenine is utilized by conversion to anthranilate (447). Inferred to be the structural gene for the alpha subunit of the anthranilate synthetase complex (546). The gene product catalyzes anthranilate synthesis with ammonia but not with glutamine as the amino donor (29). Specifies anthranitate synthetase (glutamine linked) in collaboration with trp-1 in trifunctional trp-1+-trp-2+ enzyme aggregate (181, 259) (Fig. 11); see trp-1. Nonsense allele used to isolate supersuppressors (954) and to study enzyme complex restored by supersuppressors (183).	VIR	B