FGSC #4012

Reporting Genes: cyh-1

Species: crassa

Allele: KH52(r)

Alternate Strain Number: M435 A

Mutagen: S

Depositor: EK

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: SL8

Opposite Mating Type: 4013

ref1: Kafer 1982. Neurospora Newsl. 29:41-44, https://doi.org/10.4148/1941-4765.1645

ref2: Kafer 1981 Mutation Res 80:43-64, https://doi.org/10.1016/0027-5107(81)90176-7

Genes

Locus	Cultural Requirements	Link Group	Type
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B

Neurospora Crassa Wikipedia