FGSC #3964

Reporting Genes: en(am)-1

Species: crassa

Allele: no#

Alternate Strain Number: 10i

Depositor: JRF

Linkage Group: VR

Mating Type: a

Species Number: 10

ref1: Fincham 1981 Neurospora Newsl 28:11, https://doi.org/10.4148/1941-4765.1651

Genes

Locus	Cultural Requirements	Link Group	Type
en(am)-1	VR. Between am (8%) and inl (1%). Linked to gln(1%). (122, 339, 345)In en(am)-1 am double mutants, en(am)-1 blocks the adaptation of am on minimal medium without a source of amino nitrogen (345). The double mutants are inhibited by ammonium and grow adequately only when glutamate is the sole nitrogen source. The en(am)-1single mutant grows well on minimal medium, but is unable to use, as the sole nitrogen source, proline (122), methionine, alanine, isoleucine, valine, urocanate, hypoxanthine, uridine, urea, or bovine serum albumin (184). It is relatively resistant to p-fluorophenylalanine and ethionine and completely resistant to 0.02 M glycine. These properties cosegregated with en(am)-1 in all isolates tested (339). Glutamate synthase (GOGAT) is normal (293). The single mutant is scored by using minimal medium with proline as the sole nitrogen source (122) or (better) by using 0.2 mM p-fluorophenylalanine (339). Name changed from i (inhibitor) (293).	VR	B

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