FGSC #3915

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Reporting Genes: thi-3 met-7

Species: crassa

Allele: 18558 NM251

Alternate Strain Number: 7158

Depositor: DDP

Linkage Group: VIIR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
met-7	VIIR. Right of qa-2 (<1%), ars (<1%), and the centromere (one second-division ascus in several hundred). Left of met-9 (10[-4]) and wc-1 (1 to 4%) (146, 725; M.E. Case, personal communication). (718; M.K. Allen, cited in references 718 and 789) Uses cystathionine, homocysteine, or methionine (718; N. H. Horowitz, cited in reference 1180). Lacks cystathionine-gamma-synthase (547) (Fig. 17). This enzyme is also lacking in the mutant met-3 (547). See met-3 for regulation. Apparently contiguous with met-9by coconversion. Flanking markers are recombined in most met-7+ met-9+ recombinants (725). Functionally distinct from the mutant met-9, which has active cystathionine-gamma-synthase (547) but cannot use homocysteine. No mutants lacking both functions have been isolated. Allele NM251 is suppressible by supersuppressor RN33 (same as ssu-1?) (725). Allele K79 is inseparable from reciprocal translocation T(I;VII)K79 (808).	VIIR	B
thi-3	VIIL. Between nic-3 (9 to 18%) and T(T54M50) (808, 812, 816). (482). Uses thiamine or thiazole (1059). Does not undergo growth adaptation on minimal medium. Growth on minimal medium is leaky at first, but becomes tight with exhaustion of endogenous thiazole (302), so scoring is best done late.	VIIL	B