FGSC #3606

Reporting Genes: erg-4;pan-2

Species: crassa

Allele: UVC53;Y153M66

Depositor: MG

Linkage Group: IR;VIR

Mating Type: A

Species Number: 10

ref1: Grindle Molec gen Genet 130:81-90 1974, https://doi.org/10.1007/bf00270520

ref2: Grindle and Farrow MGG 165:305-308, 1978, https://doi.org/10.1007/bf00332531

Genes

Locus	Cultural Requirements	Link Group	Type
erg-4	IR. Linked to al-1 (10%) (PB).The cell membrane is deficient in ergosterol, conferring slight resistance to nystatin. Lacks C24 (zymosterol)-methyl transferase. Accumulates zymosterol (Fig. 13.). Infertile as female (419). Slow growth, colonial at 34 C, spreading at 25°C (PB)	IR	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B

Neurospora Crassa Wikipedia