FGSC #3579

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Reporting Genes: fl;Sk-3 (Sk from N. intermedia)

Species: crassa

Allele: P;no#

Alternate Strain Number: 3167

Depositor: DDP

Linkage Group: IIR;IIIC

Mating Type: A

Species Number: 10

Opposite Mating Type: 3580

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fl	IIR. Between ace-1 (5 to 11%) and trp-3 (3%) (816, PB). (613)No macroconidia (609). Highly fertile (612). Used routinely as the female parent in tests for chromosome rearrangements and for mating type (e.g., reference 801). The flsingle mutant produces few microconidia when dry; when wetted, sufficient microconidia are produced to have been used in early irradiation and mutation studies (614, 915); large numbers can be obtained under certain conditions; see reference 893. pe fl (46, 700) and fl;dn (806) double mutants produce abundant microconidia; the latter combination is highly fertile when homozygous. Photograph of microconidial formation (774); see also reference 893. Nuclear numbers in microconidia (46, 64, 478). Wall analysis (207). Immunoelectrophoretic pattern (784). Paradoxical high alcoholic glycolysis on nitrate medium (80). Deficiency of isocitrate lyase on acetate medium; see citations in reference 1088. When fl A and fl a strains are inoculated separately on crossing medium in plates, a double line of perithecia forms where they meet, similar to that accompanying barrage in Podospora (410, 414). fl ascospores from certain fl x fl+ crosses often germinate spontaneously (1127; N. B. Raju, personal communication). Allele C-1835 was called acon (717, 812).	IIR	B
Sk-3	Near the centromere. The killer allele Sk-3K suppresses crossing over in the interval r(Sk-2)-1-leu-1 (29% in controls). Recombines with his-7 (11%) but not with leu-1 (0/72) or acr-7 (0/60,000) and only 3/19,000 with acr-2. (1092; B.C. Turner, personal communication) Origin and characteristics resemble those of Sk-2, q.v. However, Sk-3K strains are sensitive to killing by Sk-2K and vice versa. The killer allele was found in N. intermedia, introgressed into N. crassa. Most wild-type strains of both species are sensitive, but resistant strains of N. intermedia have been found. Strains resistant to Sk-3K are not necessarily resistant to Sk-2K (857, 1092).		B