FGSC #3519

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Reporting Genes: chol-2 ylo-1 ws-1

Species: crassa

Allele: 47904(t) Y30539y RP99

Alternate Strain Number: 6122

Depositor: DDP

Linkage Group: VIL VIL VIR

Mating Type: A

Species Number: 10

Opposite Mating Type: 3520

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
ws-1	VIR. Right of trp-2 (38%) (822). Delayed ascospore maturation; autonomous. Ascospores fail to darken or do so slowly. Black spots appear on some ws-1ascospores. In aged crosses, a few percent of the ws-1 ascospores darken and are capable of germination. Fertile and prototrophic, with normal tyrosinase activity vegetatively. Photograph of asci. (822). Second-division segregation frequencies may be as high as 80 to 96% (586, 822), providing evidence for chiasma interference.	VIR	B