FGSC #3484

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Reporting Genes: csp-1;cel bd

Species: crassa

Allele: UCLA37;R2366o no#

Alternate Strain Number: 2303

Depositor: SB

Linkage Group: IL;IVR IVR

Mating Type: A

Species Number: 10

ref1: Brody & Martins J. Bact. 137:912-915 1979, https://doi.org/10.1128/jb.137.2.912-915.1979

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
csp-1	IL. Between arg-3 (1%) and the T(39311) right breakpoint (972, PB). Conidia fail to separate and become airborne. Photograph (972). Recessive. Cultures on agar readily scored by the "tap test." In water, conidia are freed at 1/10 the wild-type concentration (972). Used in connection with bd for study of circadian rhythms (e.g., reference 114). Useful in student laboratories to avoid contamination (966). Carotenoids tend to be yellowish in young cultures (PB).	IL	B
bd	Because dense bands of conidia are produced on appropriate solid medium at intervals of about 24 hr (1792, 1794), the mutant has been used extensively to study circadian rhythms (239, 560, 621, 1382, 1794). bd has no effect on the underlying clock mechanism, but allows the visible expression of rhythm (622). Grow rate is about 70% that of the wild type (232). Conidiation is enhanced, even on slants (232). CO2 inhibits conidiation and, thus, inhibits banding; bd is much less sensitive than wild type to this effect of CO2 (1792). Biotin starvation leads to a phenocopy in wild type and to increased persistence of banding in bd (2206). Originally identified in a bd; inv strain called "timex" (1791). bd alone is sufficient to cause banding (1794). Used to study conidiation under nonstarvation conditions (1820). Used in a study of morphological differentiation patterns such as concentric rings and radial zonations (528). Expression is affected by changing the concentrations of agar, sugar, and salts. Conidial scatter is eliminated in the double mutant bd; csp (239). Conveniently scored by conidial banding on agar in long tubes or large plates at 25oC in constant dark or in a dark-light cycle, but not in constant light (1791).	IVR	B
cel	IVR. Linked to pan-1 (1%) and cot-1 (0/17) (812). Requires saturated fatty acids. Defect attributed to impaired chain elongation (455). Deficient in the fatty acid synthetase complex (317). increased sensitivity to oligomycin (L.R. Forman and S. Brody, cited in reference 293), Tween 20 provides a convenient supplement. Requirement is "leaky" at 21 to 22°C (317) but not at 34°C (812). Used to change fatty acid composition (113) and make the circadian clock sensitive to fatty acids and temperature (114, 649). Temperature compensation of the clock is lacking in celmutants (650). Used to study membrane lipid-phase transitions and electrical properties (363, 364). Used to incorporate photolabile azido fatty acid probes for membrane studies (176). Called ol (oleic acid) (812) and fas (fatty acid synthesis) (317). Initial report of oleic acid utilization (812) was incorrect (455), probably because of impurities.	IVR	B