FGSC #3430

Reporting Genes: sor(T9);ad-7

Species: crassa

Allele: T9M150;Y186M423

Depositor: TI

Linkage Group: IL;VR

Mating Type: A

Species Number: 10

ref1: Murayama & Ishikawa 1973 J Bacteriol 115:796-804, https://doi.org/10.1128/jb.115.3.796-804.1973

Genes

Locus	Cultural Requirements	Link Group	Type
ad-7	VR. Right of cot-2 (4%). Left of ro-4 (4%) and pab-2(8%). (158, 156). (687) Uses adenine or hypoxanthine (682). Lacks phosphoribosylpyrophosphate amidotransferase, the first enzyme in de novo purine biosynthesis (525) (Fig. 8). Ascospores from homozygous ad-7 x ad-7 crosses are white (allele Y175M256). Strains carrying allele P73Bl7l(t) are temperature sensitive.	VR	B
sor(T9)	IL. Between mt (6%) and the centromere (5%) (710). Resistant to colonializing action of sorbose at 25°C but not at 35°C (712). Low glucoamylase activity. Extracellular amylase activity <0.5 that of the wild type. Slow growth; osmotic sensitivity comparable to that of os-1mutants. High extracellular acid phosphatase activity (710). Enhances growth of gpi strains on glucose or sucrose; used to obtain mutants defective in glucosephosphate isomerase (711). Obtained by plating mutagenized conidia in medium containing starch and observing cleared zone around colonies. The glucoamylase, osmotic, and sorbose resistance properties cosegregated in 101 isolates (710). Formerly called T9 (710, 711), gla(glucoamylase) (50), and amy (325). Possibly allelic with sor-4 or sorr-15 or with both (560). Dissimilar in morphology to os-4 strains (PB). Linked to regulatory gene exo-1, which has not been tested for allelism but is stated to be on the other side of mtfrom sor(T9); see exo-1. (T in the allele number signifies Tokyo, not translocation.)	IL	B

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