FGSC #3366

Reporting Genes: ipm-1+;ipm-2+;leu-4

Species: crassa

Allele: no#;no#;R59

Alternate Strain Number: leu-4 F84 ip

Mutagen: UV

Depositor: SRG

Mating Type: a

Species Number: 10

Genetic Background: M

ref1: Reichembecher et al 1978 J Bact 133:802-810, https://doi.org/10.1128/jb.133.2.802-810.1978

ref2: https://doi.org/10.1128/jb.133.2.794-801.1978

Genes

Locus	Cultural Requirements	Link Group	Type
leu-4	IL. Right of T(OY321); hence, of cyt-1. Left of cys-5(<<1%) (1125; D.D. Perkins, N.B. Raju, and E.G. Barry, in preparation). (429)Requires leucine, Structural gene for alpha-isopropylmalate synthetase (426, 427, 432) (Fig. 15). Feedback-negative mutants (426, 427). Hybrid synthetases with altered properties (432). Complementation between alleles (432, 1125).	IL	B
ipm-1	Unmapped. Unlinked to ipm-2 or leu-4.Able to use alpha-isopropylmalate to support growth of leu-4 mutants and for induction of alpha-isopropylmalate isomerase and beta-isopropylmalate dehydrogenase, in contrast to ipm+ strains, which are unable to take up this intermediate (870, 871).		B
ipm-2	Unmapped. Unlinked to ipm-1 or leu-4.Improves alpha-isopropylmalate uptake by the mutant ipm-1 in supporting growth of the mutant leu-4(870, 871). By itself ipm-2 is not very effective in promoting permeability of alpha-isopropylmalate.		B

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