FGSC #3304

Reporting Genes: [cni-3];leu-1

Species: crassa

Allele: 1;??

Alternate Strain Number: cni-3

Depositor: DLE

Linkage Group: --;VR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: 1978 Degn et al FEBS 11th Meeting Copenhagen pp 21-29

Genes

Locus	Cultural Requirements	Link Group	Type
{cni-3}			B
leu-1	IIIR. Between ad-4 (1 to 5%) and his-7 (8%) (219, 578, 815, 1052). (868)Requires leucine (867, 868). Lacks beta-isopropylmalate dehydrogenase (426) (Fig. 15). Accumulates alpha-isopropylmalate and beta-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as inducer (427). Resistant to aminotriazole (D. D. Perkins, unpublished data). Female sterile (O. M. Mylyk, personal communication). Used to study reversion and competition in heterokaryons (901).	IIIR	B

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