FGSC #3259

Reporting Genes: ad-3B

Species: crassa

Allele: 2-17-137

Alternate Strain Number: 2-17-137

Mutagen: NA

Depositor: FJD

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: SL

Opposite Mating Type: 3846

ref1: Barnett & de Serres Genetics 48:717-723 1963 B25a, https://doi.org/10.1093/genetics/48.5.717

ref2: de Serres et al Mut Res 4:415-424 1967 XD46, https://doi.org/10.1016/0027-5107(67)90004-8

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B

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