FGSC #3238

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Reporting Genes: fs-2;pan-2;nic-3

Species: crassa

Allele: 10-4(t);Y153M96;Y31881

Alternate Strain Number: F120

Mutagen: S

Depositor: OMM

Linkage Group: --;VIR;VIIL

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Mylyk & Thelkeld 1972 NN19:21, https://doi.org/10.4148/1941-4765.1871

ref2: Mylyk & Thelkeld 1974 Genet Res 24:91-102, https://doi.org/10.1017/s001667230001510x

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
fs-2	Tentatively II; probable loose linkage to fs-1 (732) and cot-5 (14/48) (PB).No perithecia when used as the female. Fertile as the male. Abnormal morphology, somewhat colonial. Grows at 25 C, but not at 34°C. Recessive in heterokaryons. Complements fs-1, -3, 4, -5, -6, and -n. (O.M. Mylyk, personal communication)	II?	B
nic-3	VIIL. Right of spco-4 (1%) and do (3%). Left of thi-3 (9 to 27%) and csp-2 (16 to 22%) (539, 812, 816, PB). (M.K. Allen, cited in references 718 and 789) Uses nicotinic acid, nicotinamide, 3-hydroxyanthranilic acid, 3-hydroxykynurenine, or high concentrations of quinolinic acid (96, 1168). Accumulates alpha-N-acetylkynurenine; blocked in conversion of kynurenine to 3-hydroxykynurenine (1168) (Fig. 18). Pyridine nucleotide levels (111). Pathway from tryptophan to nicotinic mononucleotide, showing sites of gene action (96, 100, 368, 1168). The enzymatic reactions between 3-hydroxyanthranilate and nicotinic mononucleotide have not been demonstrated directly in Neurospora.	VIIL	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B