FGSC #3237

Reporting Genes: fs-2;pan-2

Species: crassa

Allele: 10-4(t);Y153M96

Alternate Strain Number: F119

Mutagen: S

Depositor: OMM

Linkage Group: --;VIR

Mating Type: a

Species Number: 10

Genetic Background: M

ref1: Mylyk & Thelkeld 1972 NN19:21, https://doi.org/10.4148/1941-4765.1871

ref2: Mylyk & Thelkeld 1974 Genet Res 24:91-102, https://doi.org/10.1017/s001667230001510x

Genes

Locus	Cultural Requirements	Link Group	Type
fs-2	Tentatively II; probable loose linkage to fs-1 (732) and cot-5 (14/48) (PB).No perithecia when used as the female. Fertile as the male. Abnormal morphology, somewhat colonial. Grows at 25 C, but not at 34°C. Recessive in heterokaryons. Complements fs-1, -3, 4, -5, -6, and -n. (O.M. Mylyk, personal communication)	II?	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B

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