FGSC #3232

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Reporting Genes: cni-1 inl

Species: crassa

Allele: 1;89601

Alternate Strain Number: CNE-1

Mutagen: UV

Depositor: DLE

Linkage Group: --;VR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Edwards and Unger 1973 J. Bacteriol 114:164-168, https://doi.org/10.1128/jb.114.1.164-168.1973

ref2: Klein et al. J. Biol Chem 250:5852-5858 1975, https://doi.org/10.1016/S0021-9258(19)41131-9

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cni-1	Unmapped chromosomal gene. Not in V (309). No cyanide-sensitive or antimycin A-sensitive respiration in the first 24 h of growth. Also, initially insensitive to salicyl hydroxamic acid. But salicyl hydroxamic acid and cyanide together inhibit (309). Cyanide-insensitive respiration and the cytochrome c level decrease markedly in the late log phase and stationary phase, whereas the cytochrome aa3 level increases rapidly (559). As shown by electron microscopy, mitochondria are defective in the early log phase, but later resemble the wild type (558). Behaves as a cold-sensitive ribosome assembly mutant. Small subunits are not assembled at low temperatures; the respiratory differences between early and late growth noted above were found at 30°C, and normal aa3 production occurred throughout growth at 37°C (548). Electron spin resonance data (308). Selected by failure to reduce tetrazolium in overlay after inositol-less death enrichment (310).		B
inl	VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.	VR	B