FGSC #3230

Reporting Genes: pan-2;qa-2

Species: crassa

Allele: B23;204

Alternate Strain Number: 204-23-22A

Depositor: MEC

Linkage Group: VIR;VIIR

Mating Type: A

Species Number: 10

Genetic Background: SL

ref1: Case et al. J Bacteriology 129:166-172 1977, https://doi.org/10.1128/jb.129.1.166-172.1977

Genes

Locus	Cultural Requirements	Link Group	Type
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B
qa-2	VII. In the qa cluster between qa-4 and met-7 (0.02%) (146). (884) Lacks catabolic dehydroquinase (148, 517, 884). Unable to use quinate or shikimate as the sole carbon source in the presence of aro-9, which results in the absence of biosynthetic dehydroquinase (885) (Fig. 21). aro-9;qa-2+ strains grow on minimal medium without supplement. qa-2 is conveniently scored as an aro auxotroph when aro-9 is present. qa-2+ cloned in pBR322 (pVK57) is expressed constitutively from its own promoter in E. coli(23, 388, 451); has been returned to Neurospora chromosomal sites by transformation (150). Allele M246 is stable (150).	VII	B

Neurospora Crassa Wikipedia