FGSC #3220

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Reporting Genes: T(I->II)39311,ser-3;arg-1;tol

Species: crassa

Allele: 39311 47903 B369 N83

Alternate Strain Number: 12-1248

Depositor: DDP

Linkage Group: IL;IIIR;IVR

Mating Type: a

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
arg-1	Uses arginine but not precursors.	IL	B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B
ser-3	IL. Right of cys-5 (<1%). Left of un-3 (<1%) and In(NM176) (8I6, 1093, PB). Uses serine and grows less well on formate. Also grows fairly well on a combination of adenine, methionine, tryptophan, and lysine. Does not grow on casein hydrolysate (290). No or little response to glycine (D.D. Perkins, unpublished data). Deficient in phosphoserine phosphatase activity (70). Inhibited by leucine. Scorability good, but vigor and leakiness vary markedly in different isolates. Homozygous crosses give mostly white ascospores (816; D.D. Perkins, unpublished data). Alleles, 47903 and JBM5.	IL	B
T(I->II)39311	Translocation		B