FGSC #3190

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Reporting Genes: T(IV->VI)ALS159,trp-4 met-2;ylo-1

Species: crassa

Allele: ALS159 Y2198 K43 Y30539y

Alternate Strain Number: 46-1149

Depositor: DDP

Linkage Group: IVR;VIR;IVR IVR;VIL

Mating Type: A

Species Number: 10

ref1: Perkins & Barry 1977 Advances Genet 19:133-285, https://doi.org/10.1016/s0065-2660(08)60246-1

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Genes

Locus	Cultural Requirements	Link Group	Type
met-2	IVR. Between trp-4 (6%) and pan-1 (4%) (719). Linked to ilv-3(0/129) (354, 579). Uses methionine or homocysteine; accumulates cystathionine (469). Lacks cystathionase II (353) (Fig. 17). Fine-structure map (720, 724). Complementation map (719). Used in major studies of intralocus recombination and its polarity (720, 724).	IVR	B
trp-4	IVR. Between his-5 (3 to 7%) and leu-2 (1 to 2%) (633, 991). (47). Uses tryptophan or indole (750). Deficient in anthranilate phosphoribosyl transferase (1126) (Fig. 11). Scorable by blue fluorescence (anthranilate) in medium under long-wave UV after 2 to 5 days of growth on minimal medium plus indole (10 µg /ml), 34°C. Initial stocks of the first trp-4 mutant were inhibited by suboptimal concentrations of tryptophan (750), but derivatives have been obtained that are free of this problem (909).	IVR	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B
T(IV->VI)ALS159	Translocation		B