FGSC #3178

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Reporting Genes: T(I->V)AR190,nic-2 al-2 lys-1

Species: crassa

Allele: AR190 43002 MN58 66202

Alternate Strain Number: 15-738

Depositor: DDP

Linkage Group: IR;VR;IR IR IR

Mating Type: A

Species Number: 10

Opposite Mating Type: 3179

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
lys-1	V. Right of caf-1 (4 to 14%). Left of cyt-9 (5%) and at (1 to 20%) (817; K.S. Hsu, personal communication; PB). (403)Uses lysine, alpha-aminoadipic acid, or epsilon-hydroxynorleucine (alpha-amino-epsilon-hydroxycaproic acid) (399, 400, 684, 1087). Accumulates homocitrate on limiting lysine concentrations (464) (Fig. 16). Fine structure and complementation between alleles (8). Initial allele: 33933.	V	B
nic-2	IR. Between ad-3B (4%) and ace-7 (4 to 7%) (271, 578). (482) Grows on nicotinic acid, nicotinamide, or high concentrations of quinolinic acid (97, 1168). Cannot use kynurenine, hydroxykynurenine, or hydroxyanthranilic acid (96, 1168). Accumulates 3-hydroxyanthranilic acid (96) (Fig. 18). Aging cultures accumulate red-brown pigment in the medium. Used to study intralocus recombination (908). Translocations T(4540) and T(S1325)are inseparable from nic-2 (808, 908, 911).	IR	B
T(I->V)AR190	Translocation		B