FGSC #3135

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Reporting Genes: T(I->VI)NM103,cyh-1 al-1y arg-13 R

Species: crassa

Allele: NM103 KH52(r) ALS4 RU3 35408R

Alternate Strain Number: 16-981

Depositor: BCT

Linkage Group: IR;VIR;IR R R R

Mating Type: a

Species Number: 10

ref1: Turner, B.C. 1977 Genetics 85:439-460

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-1y	No carotenoids produced.	IR	B
arg-13	Responds well to arginine or citrulline and poorly to ornithine.	IR	B
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B
T(I->VI)NM103	Translocation		B
R	IR. Right of T(NM169d) and aro-8 (4%). Left of un-18(11%) (1093). (H.R. Cameron, personal communication) All eight ascospores of heterozygous R/+ asci are round rather than ellipsoid. R is thus nonautonomous in ascospores and dominant in the ascus (690). Ascospores are round even in nonlinear asci (1008; D.D. Perkins, unpublished data). Usually two germination pores are formed, but sometimes one (1008). Photograph (1008). Vegetative morphology abnormal, somewhat resembling that of pe mutants. Initial growth on slants is concentrated around the inoculation point. The vegetative morphology is recessive in heterozygous duplications, as from T(NM103) (1091). Female sterile, with no perithecia, but R x R crosses can be accomplished if R is heterokaryotic in the female parent (A.M. Srb, personal communication). Used in studies of duplication instability (1091) and autonomy of ascospore development (529). Allelic recurrences obtained (1008; A.M. Srb, personal communication). For other genetically determined round ascospores, see reference 59. Called Rsp (1008), but the original symbol R has priority. (rsp has been used for cytoplasmically determined respiration-deficient mutants [890].)	IR	B