FGSC #3112

Add to Cart

Reporting Genes: (fmf-1;tol pan-1 A + tol trp-4 a)

Species: crassa

Allele: PB-J6;N83 5531 + N83 Y2198

Alternate Strain Number: 772

Depositor: TEJ

Linkage Group: IL;IVR + IIIR;VR

Mating Type: B

Species Number: 10

ref1: 1976 Johnson Genetics 83:36-37 (Abstr)

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
a			B
fmf-1	I. Between mt (2 to 15%) and cr-1 (2%). Linked to arg-1(531).Perithecial development is blocked 15 h after fertilization, before meiosis, when fmf-1 is present either in the female or male parent. Perithecia attain only 40% normal diameter. Recessive in heterokaryons and can be crossed as one component of a heterokaryon, either as female or as male. Female fertility is also restored in mixed mating type (fmf-1 A + fmf+a) heterokaryons that are homokaryotic for tol(531). Called PBJ6 (527).	I	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B
trp-4	IVR. Between his-5 (3 to 7%) and leu-2 (1 to 2%) (633, 991). (47). Uses tryptophan or indole (750). Deficient in anthranilate phosphoribosyl transferase (1126) (Fig. 11). Scorable by blue fluorescence (anthranilate) in medium under long-wave UV after 2 to 5 days of growth on minimal medium plus indole (10 µg /ml), 34°C. Initial stocks of the first trp-4 mutant were inhibited by suboptimal concentrations of tryptophan (750), but derivatives have been obtained that are free of this problem (909).	IVR	B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B
A			B