FGSC #3055

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Reporting Genes: T(V;VI)T54M117 un

Species: crassa

Allele: T54M117

Alternate Strain Number: 56-1204

Depositor: DDP

Linkage Group: VR VI

Mating Type: A

Species Number: 10

Opposite Mating Type: 3056

ref1: Perkins & Barry 1977 N.N. 24:12-13, https://doi.org/10.4148/1941-4765.1748

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
T(V;VI)T54M117	Translocation		B
un	Unknown function. Temperature-sensitive (heat-sensitive) conditional mutants, irreparable by supplementation at the restrictive temperature (usually 34 to 37 C). Originally referred to as "unknown requirement" on the initial hypothesis that such mutants would prove to be auxotrophs. Several heat- sensitive genes have been sufficiently characterized to be assigned more specific names than un (e.g., ndc, rip, psi, eth-1, and fs-2). At least some unmutants are deficient in amino acid transport (543, 1075). For most un loci, however, little is known of the molecular or cellular basis. Because of their map locations, several are useful as genetic markers. Scoring may require growth comparisons at two temperatures, preferably with small conidial inocula. Some heat-sensitive mutants with altered morphology at restrictive temperature are called cot or scot. Temperature-sensitive auxotrophs with certain requirements have sometimes been classed initially as un because complex complete medium is either inadequate or inhibitory. Thus, complex-medium-irreparable temperature-sensitive mutants no. 3, 13, and 14 of reference 507 proved to be temperature-sensitive thr auxotrophs; no. 6, 20, and 30 were his; and no. 19, 24, and 35 were asn(T. Ishikawa, personal communication). Many un mutants do not achieve normal growth rates even at the permissive temperature (usually 25 C). Genes un-1 to un-8 were assigned locus numbers in reference 813. "T" in allele numbers of several un mutants designates Tokyo, not translocation.		B