FGSC #3001

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Reporting Genes: al-2 pan-1;fpr-5

Species: crassa

Allele: 15300;5531;no#(r)

Alternate Strain Number: J192 (a) J19

Depositor: JAK

Linkage Group: IR IR;IVR

Mating Type: A

Species Number: 10

Opposite Mating Type: 3002

ref1: Kinsey Neurospora Newsletter 22:20 1975, https://doi.org/10.4148/1941-4765.1792

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
fpr-5	1. Left of al-2 (25%) (550). Resistant to p-fluorophenylalanine, but not to 4-methyltryptophan. Isolated in the wild type. Not tested for amino acid uptake. Scored on solid medium containing 10 µg of p-fluorophenylalanine or 60 µg of 4-methyltryptophan per ml. (550)	I	B
pan-1	IVR. Between ad-6 (1 to 2%) and cot-1 (2 to 3%) (633, 692, PB). (482). cel, col-1, int, pho-3, and thi-5 all appear to be closely linked in this crowded region. Requires intact pantothenic acid for growth under standard conditions. Able to synthesize both precursors, beta-alanine and pantoyl lactone (1058). Ability to synthesize pantothenic acid from beta-alanine plus pantoyl lactone is demonstrable in vitro but not in vivo unless cultures are aerated (1111, 1113, 1114). Unlike pan-2, pan-1 has no effect on ascospore ripening in heterozygous crosses. Called group A. For alleles see reference 138.	IVR	B