FGSC #2994

Reporting Genes: nit-4 (N. intermedia)

Species: crassa

Allele: no#

Alternate Strain Number: 4202

Depositor: DDP

Linkage Group: IVR

Mating Type: a

Species Number: 10

Genetic Background: SL3

Opposite Mating Type: 2993

ref1: Blakely & Srb 1962 NN 2:5-6, https://doi.org/10.4148/1941-4765.1043

Genes

Locus	Cultural Requirements	Link Group	Type
nit-4	IVR. Right of pyr-1 (1 to 6%). Probably right of col-4(2%). Left of pan-1 (6 to 27%) (1000, PB). (94) Cannot use nitrate or nitrite as a nitrogen source, but uses ammonia and amino acids (94). Regulator for induction by nitrate of nitrate reductase and nitrite reductase (1080). Allele nr15, called nit-5 (1000), is phenotypically identical to other nit-4 alleles; shown to be allelic by failure to complement or recombine (0 prototrophs per 2,080 progeny) (1080). Original allele was discovered in a wild isolate of N. intermedia from Borneo and introgressed into N. crassa (94).	IVR	B

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