FGSC #2977

Reporting Genes: nit-6 pan-2

Species: crassa

Allele: OP4 B36

Alternate Strain Number: O-P-4A

Depositor: GJS

Linkage Group: VIL VIR

Mating Type: A

Species Number: 10

ref1: Chang J Bacteriol 123(2):755-758 1976, https://doi.org/10.1128/jb.123.2.755-758.1975

ref2: 1969 CJ Dyer MSc McMaster University

Genes

Locus	Cultural Requirements	Link Group	Type
nit-6	VIL. Right of chol-2 (6%). Left of ser-7 (10%) and ad-8(17%) (PB; O.C. Yoder, personal communication). Unable to use nitrate or nitrite as a nitrogen source (185). Lacks nitrite reductase (185) (Fig. 19), which is subject to positive nitrogen metabolite repression (186). Affected by nit-2 and MS5 regulator genes (838, 1076a) (see nmr-1). Used to study repression of nitrate reductase (26) and nonenzymatic reduction of nitrite (185). Induced by nitrite (198).	VIL	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B

Neurospora Crassa Wikipedia