FGSC #2975

Reporting Genes: In(IL->IR)H4250,tol

Species: crassa

Allele: H4250;N83

Alternate Strain Number: 11-1082

Depositor: DDP

Linkage Group: IL IR;IVR

Mating Type: a

Species Number: 10

Opposite Mating Type: 1947

ref1: Newmeyer 1970 Can J Genet Cytol 12:914-916, https://doi.org/10.1139/g70-115

Genes

Locus	Cultural Requirements	Link Group	Type
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B
In(IL->IR)H4250			B

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