FGSC #2967

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Reporting Genes: ff-1;ylo-1;inl

Species: crassa

Allele: 744;Y30539y;83201(t)

Alternate Strain Number: 33

Depositor: JBC

Linkage Group: IIR;IVL;VR

Mating Type: A

Species Number: 10

ref1: Courtright 1975 J Bact 124:497-502, https://doi.org/10.1128/jb.124.1.497-502.1975

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ff-1	IIR. Between aro-1 (5%) and un-20 (4%) (1052). (1053)Female sterile with no protoperithecia (1052, 1053). Enhanced glycerol utilization; aconidial on liquid glycerol medium. Good conidiation, but reduced aerial hyphae and heavy surface growth on sucrose minimal slants. Growth on glycerol reduces levels of both pyruvate dehydrogenase and dihydrolipoyl transacetylase (211). Effect of carbon source studied (1078). Conveniently scored by failure to produce protoperithecia on small slants of synthetic cross medium (7 days, 25 C). Allele T30 was originally recognized as specifying female sterility and was called ff-1 (1052, 1053). Allele JC744 was first characterized by glycerol utilization and called glp-3 (211).	IIR	B
inl	VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.	VR	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B