FGSC #2949

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Reporting Genes: chol-1;chol-2

Species: crassa

Allele: 34486;47904(t)

Alternate Strain Number: SCH 42

Depositor: SB

Linkage Group: IVR;VIL

Mating Type: A

Species Number: 10

ref1: Hubbard & Brody 1975 J Biol Chem 250:7173, https://doi.org/10.1016/S0021-9258(19)40925-3

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
chol-1	IVR. Linked to ad-6 (1%). probably to the right (633). (482) Requires choline (470). Also uses mono- or dimethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidylethanolamine-N-methyl transferase (222, 924). Abnormal colonial morphology on limiting choline (222). Colonies from single conidia on minimal agar medium resemble inhibited A/a duplications, with swollen hyphae and darkening in the presence of phenylalanine plus tyrosine (D.D. Perkins, unpublished data). Abnormal phospholipid composition on limited concentrations of supplement (501). Best scored late on minimal medium. Grows slightly and then stops (D.D. Perkins, unpublished data). Used to study inhibition of cytochrome-mediated respiration and of conidiation when lecithin is depleted by choline starvation (534, 535). Initial allele called 34486.	IVR	B
chol-2	VIL. Left of nit-6 (6 to 8%) (812, PB). Requires choline (471). Also uses di- but not monomethylaminoethanol (468) (Fig. 12). Deficient in S-adenosylmethionine:phosphatidyl- monomethylethanolamine methyltransferase (222, 923, 924). Strains carrying the only allele, 47904t, are leaky on minimal medium at 22°C but not at 34°C (501). Phospholipid composition is abnormal on limiting choline (501). Growth is colonial on limiting supplement at 34°C and on minimal medium at 25°C.	VIL	B