FGSC #2923

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Reporting Genes: un-5 al-2;pi;cys-10

Species: crassa

Allele: b39(t) 15300;B101;39816

Alternate Strain Number: 9223

Depositor: DDP

Linkage Group: IL R;IIL;IVL

Mating Type: a

Species Number: 10

Opposite Mating Type: 2922

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
cys-10	IVL. Left of acon-3 (1 to 6%), ace-4 (19 to 33%), and cut (28 to 37%) (578, PB). (721) Uses cysteine, cystathionine, homocysteine, or methionine, with a slight response to thiosulfate (469, 596, 721); however, E. Kafer (personal communication) found good growth on thiosulfate. Growth is better on casein hydrolysate than on methionine (D.D. Perkins, unpublished data). cys-10 chol-1double mutants grow better on methionine alone than on methionine plus choline (721). Lacks sulfite reductase, as do cys-2 and cys-4mutants (596). Formerly called met-4; see reference 721.	IVL	B
pi	IIL. Linked to ro-7 (0/75); left of cys-3 (4%) (816). Spreading growth (allele B101); no conidia. (816) Growth is better on minimal medium than on complex complete medium. The mutant pi (B101) has not been tested for 6-phosphogluconate dehydrogenase, which is deficient in strain(s) carrying putative allele col-10 (R2438) (947). See col-10regarding allelism. As a marker, pi (B101) is preferable to col-10 (R2438) because of growth rate, stability, and ease of handling. ro-7, in the same region, is preferable to both (PB).	IIL	B
un-5	IL. Right of fr (6%). Left of T(39311) and nit-2 (2%) (816, 798). (574). Unknown function. Heat sensitive, 34°C versus 23°C. Inhibited by histidine and tryptophan: osmophilic (26 to 28 C) (574, 575). Formerly called un(b39).	IL	B