FGSC #2765

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Reporting Genes: mei-3;tol

Species: crassa

Allele: N289;N83

Alternate Strain Number: N433R4, SG15

Mutagen: S

Depositor: DNP

Linkage Group: IL;IVR

Mating Type: a

Species Number: 10

Genetic Background: SL3

ref1: Newmeyer & Galeazzi Genetics 77:s48 1974 (Abstr)

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mei-3	IL. Right of arg-1 (3%). Probably right of eth-1 (1%) and arg-3 (1%). Left of the T(39311)right breakpoint; hence, left of the centromere and sn (757, 808).Homozygous barren (757). Recessive. Blocks meiosis in zygotene (860). Sensitive to UV, histidine (757, 759), mitomycin C (195), ionizing radiation, and methyl methane sulfonate (939). Sensitivity to UV and histidine is temperature sensitive; best scored at 39 C, at least for strains carrying allele N289 (757). Causes increased duplication instability (mitotic recombination, deletion, or both) (757).	IL	B
tol	IVR. Linked to trp-4 (~1%), probably to the left (755). Suppresses the vegetative (heterokaryon) incompatibility associated with mating type alleles A and a, but does not affect sexual compatibility. (tol;A+ tol;a) heterokaryons are fully compatible and stable if other het loci are homokaryotic, and A/a duplications grow normally when tol is present (755). Recessive (252); see reference 746, however, for a stable mixed-mating type heterokaryon that is (tol a + tol+ slime A). tol does not suppress the vegetative incompatibility of differing alleles at het-c or het-e(755, 803). Mutation or deletion of tol+ restores normal growth rate to slow-growing, unstable, mixed-mating-type (tol a + tol+ A) heterokaryons (252). tol is present in some isolates from nature and has arisen at least twice by mutation in laboratory stocks (755, PB; O.C. Yoder, personal communication). Double-mutant tol trp-4 stocks are convenient because the closely linked trp-4 tags the tol allele, which otherwise requires progeny tests for scoring. Used to maintain stable A + a heterokaryons, allowing the desired component to be used as the parent in a cross (746). Homozygous tolmay partially restore fertility to the mutant fmf-1 (531).	IVR	B