FGSC #2744

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Reporting Genes: arg-5;ota;aga

Species: crassa

Allele: 27947;UM728;UM906

Alternate Strain Number: arg-5,ota,ag

Depositor: RHD

Linkage Group: IIR;IIIR;VIIR

Mating Type: A

Species Number: 10

ref1: Davis et al J Bacteriol 102(2):299-305 1970, https://doi.org/10.1128/jb.102.2.299-305.1970

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ota	IIIR. Between ad-4 (15%) and tyr-1 (14%) (241). Linked to pro-4 (4%) (D.J. West, cited in Neurospora Newsl. 16:19-22, 1970). Ornithine-delta-transaminase deficient (241) (Fig. 10). Conidiates somewhat less than does the wild type (S. Brody, personal communication). Selected by ability to use exogenous ornithine as a precursor for arginine in an arg-5 arg-12s double mutant. Catabolism of ornithine (to glutamic-gamma-semialdehyde) is blocked, resulting in ornithine concentrations high enough to compensate for the low activity of the ornithine carbamyl transferase in the arg-12s mutant. The ota single mutant is prototrophic but prevents the efficient use of ornithine or arginine as the sole nitrogen source (241). Used to study flux through the arginine biosynthetic pathway (401). Used to study the utilization of endogenous versus exogenous ornithine (234). Sideramine production is completely blocked in absence of ornithine in the ota;arg-5;aga triple mutant, which is used to study iron transport (1146, 1147).	IIIR	B
aga	VIIR. Between wc-1 (2%) and arg-10 (24 to 27%) (240, 697). Presumed structural gene for arginase (240, 697) (see Fig. 10). Unable to form ornithine from arginine; arginine is thus unable to satisfy the proline requirement of pro-3 in a pro-3 aga double mutant. Prototrophic single mutants develop polyamine requirement in the presence of arginine. This is due to feedback inhibition of ornithine biosynthesis by arginine, combined with a catabolic block in ornithine formation from arginine (240). Siderophore production is severely reduced in the absence of ornithine in the triple mutant aga arg-5 ota, which has been used to study iron transport (1146, 1147) and to obtain mutants defective in siderophore uptake (G.W. Charlang and N.P. Williams, personal communication); see sit.	VIIR	B
arg-5	Uses ornithine, citrulline or arginine.	IIR	B