FGSC #2737

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Reporting Genes: glt;leu-2

Species: crassa

Allele: D240(R);37501

Alternate Strain Number: 193

Mutagen: UV

Depositor: GAM

Linkage Group: --;IVR

Mating Type: a

Species Number: 10

Genetic Background: M

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
glt	Unmapped.Unable to transport oligopeptides necessary to support growth of specified amino acid auxotrophs (1155). Has only 10% of the wild-type uptake rate (1154). (Oligopeptide uptake system transports tri-, tetra-, and pentapeptides, but not di- or higher than pentapeptides.) Obtained, using tys, by selecting mutants resistant to glycyl-L-leucyl-L-tyrosine but still sensitive to tyrosine (1155). See reference 1151 for a review of peptide uptake.		B
leu-2	IVR. Between trp-4 (2%) and ilv-3 (4%) (579, 991). (D.C. Regnery, cited in reference 633)Requires leucine (867, 868). Structural gene for isopropylmalate isomerase (432, 871) (Fig. 15). Altered heat inactivation of hybrid enzymes (432). Structural differences of hybrid enzymes (871). Accumulates alpha-isopropylmalate (427). Synthesis of the enzyme also requires the function of regulatory gene leu-3+ and the presence of alpha-isopropylmalate, which acts as an inducer (427). Resistant to aminotriazole (D.D. Perkins, unpublished data). Alleles show intralocus complementation (424). Allele 37501 is heat sensitive (30°C versus 20 C) and is leaky at 25°C (D.C. Regnery, personal communication).	IVR	B