FGSC #2724

Add to Cart

Reporting Genes: erg-2;pan-2

Species: crassa

Allele: NAN25;Y153M66

Alternate Strain Number: Nan 25 pan A

Mutagen: NA

Depositor: MG

Linkage Group: VR;VIR

Mating Type: A

Species Number: 10

Genetic Background: M

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B
erg-2	VR. Left of inl (6%) (419). The cell membrane is deficient in ergosterol, thereby conferring slight resistance to nystatin and other polyene antibiotics (417, 418). Lacks 24(28)dehydroergosterol hydrogenase (terminal step of ergosterol synthesis) (419) (Fig. 13.). Poorly fertile as female. Good growth and conidiation.	VR	B