FGSC #2722

Reporting Genes: erg-1;pan-2

Species: crassa

Allele: uv1;Y153M66

Alternate Strain Number: UV-1 pan A26

Depositor: MG

Linkage Group: VR;VIR

Mating Type: A

Species Number: 10

ref1: Grindle & Farrow Mol gen Genet 165:305-308 1978, https://doi.org/10.1007/bf00332531

Genes

Locus	Cultural Requirements	Link Group	Type
erg-1	VR. Between pk (2%) and asn (9%) (419).The cell membrane is deficient in ergosterol, conferring strong resistance to nystatin and other polyene antibiotics (418, 419). Defective in conversion of fecosterol to episterol (Fig. 13.). Lacks fecosterol delta8,delta7-isomerase (419). Infertile as female. Slow growth, reduced conidiation. The nysrmutants of reference 699 are blocked in the same reaction, but nysr has not been tested for allelism with erg-1.	VR	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B

Neurospora Crassa Wikipedia