FGSC #2707

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Reporting Genes: cys-10 cut pyr-1

Species: crassa

Allele: 39816 LLM1 H263

Alternate Strain Number: 4190

Depositor: DDP

Linkage Group: IVL IVR IVR

Mating Type: A

Species Number: 10

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cut	IVL. Between cys-10 (28 to 37%) and fi (4 to 10%) (802; D.D. Perkins, unpublished data). Linkage to IR, shown in original cut strain HK53, was due to an unrecognized I;IV translocation (808). When an allelic cut point mutation (LLMI) became available, it was mapped in IV rather than in I and segregated independently of I markers (802). Sensitive to high osmotic pressure. Phenotype similar to that of os mutants. Scorable either by morphology or by failure to grow on agar containing 4% NaCl. Morphology approaches normal at high humidity (573). Allele HK53 is inseparable from T(I;IVL)HK53 (808).	IVL	B
cys-10	IVL. Left of acon-3 (1 to 6%), ace-4 (19 to 33%), and cut (28 to 37%) (578, PB). (721) Uses cysteine, cystathionine, homocysteine, or methionine, with a slight response to thiosulfate (469, 596, 721); however, E. Kafer (personal communication) found good growth on thiosulfate. Growth is better on casein hydrolysate than on methionine (D.D. Perkins, unpublished data). cys-10 chol-1double mutants grow better on methionine alone than on methionine plus choline (721). Lacks sulfite reductase, as do cys-2 and cys-4mutants (596). Formerly called met-4; see reference 721.	IVL	B
pyr-1	IVR. Right of psi (4%) and T(ALS159) (808, PB). Left of pdx-1 (<1 to 10%) (692). (482) Requires uracil or other pyrimidine. Lacks dihydroorotate dehydrogenase activity (133, 134) (Fig. 20). All ascospores from pyr-1 x pyr-1crosses are white if cross is on medium containing 0.1 mg of uracil per ml; they are black if the cross is on medium containing 1.0 mg/ml (632).	IVR	B