FGSC #2650

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Reporting Genes: al-2;rip-1;inl

Species: crassa

Allele: 15300 4M(t) 89601

Alternate Strain Number: 4Mts

Depositor: ML

Linkage Group: IR;IIR;VR

Mating Type: A

Species Number: 10

ref1: Loo 1975 N. N. 22:10-11, https://doi.org/10.4148/1941-4765.1785

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
rip-1	IIR. Linked to un-15 (1%); right of fl and trp-3 (6 to 9%). (PB). Conditional defect in production of 60S ribosomal subunits (622). At the restrictive temperature (37 C), RNA synthesis is affected first and then protein synthesis; 60S cytosolic ribosomal subunits are underaccumulated, and relatively little 25S rRNA is produced (618, 620, 622; P.J. Russell, personal communication). Defective ribosome biosynthesis at high temperatures is attributed to a defect in rRNA processing (622). Attains 2.4% of the wild-type growth rate at 35°C and 80% at 25°C (P.J. Russell, personal communication). Scored as an irreparable heat-sensitive un mutant (see un). Good fertility, growth, and viability make rip-1preferable to un-15 as a marker for the right end of II. The onginal strain carrying both rip-1 and inl (89601) (382, PB). was called 4M(t). The rip-1 allele in this strain was originally called 1(t).	IIR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
inl	VR. Between pho-3 (3 to 4%) and pab-1 (1 to 10%). Right of al-3 (362, 397, 1036). (482)Requires inositol (65). Lacks D-myoinositol-1-phosphatase (1142). Lack of glucocycloaldolase found by Pina and Tatum (826) is attributed by Williams (1142) to drastic repression of glucocycloaldolase by the concentration of inositol used for growth. Growth is colonial on low levels of inositol (367). Tends to extrude dark pigment into the medium when grown on suboptimal inositol. Composition of phospholipids and cell walls is abnormal on limiting inositol (367, 439, 440, 501). Inhibited by hexachlorocyclohexane (366, 457, 931). Conidia are subject to death by unbalanced growth on minimal medium (1028, 1033), a property exploited for mutant enrichment ("inositol-less death") (606, 647) because double mutants are at a selective advantage. Heat-sensitive allele 83201 is especially useful for mutant enrichment (832, 1043). Used in the first experiments reporting transformation of Neurospora by N. crassaDNA (677, 679) and reported to be efficient as a recipient in absence of inositol (1162). Used to study glucose (917) and sulfate (641) transport systems. Used extensively for studying induced reversion (392). Used for studying the mechanism of inositol-less death (647, 702), mutagenicity of ferrous ions, and regulation of mitochondrial membrane fluidity; for a review, see reference 702. Spontaneous reversion rates (386). Allele-specific partial suppressor (390). Allele 46802 is nonrevertable and inseparable from translocation 46802 (386, 808). Strains carrying heat-sensitive allele 83201 show slow semicolonial growth in liquid minimal medium at 25°C (641), but look normal on slants (D.D. Perkins, unpublished data). Strains carrying allele 89601 contain cross-reacting material (1183). Mutant gene exo-1 is present in the inl(89601) a stock FGSC 498 and may, therefore, be present in stocks of mutants derived by inositol-less death. (See references 194, 325, and 1027). Called inos.	VR	B