FGSC #2609

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Reporting Genes: Has been replaced by strain number: 4607

Species: crassa

Allele: Pm-22(r);no#

Alternate Strain Number: 35

Depositor: EWR

Linkage Group: IVR;IIL

Mating Type: A

Species Number: 10

ref1: 1974 E Wong Florida State University Masters Thesis

ref2: https://doi.org/10.4148/1941-4765.1787

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
mtr	IVR. Between pdx-1 (2%) and col-4 (1%) (101, 1017).Resistant to 4-methyltryptophan and p-fluorophenylalanine. pmn (= Pm-N, pm n), selected by resistance to p-fluorophenylalanine, has been shown to be alletic with mtr(R. Sadler and S. Ogilvie-Villa, personal communication; see also reference 248). Defective in transport of neutral aliphatic and aromatic amino acids via amino acid transport system I (as defined in reference 777) (248, 602, 1017, 1152). Causes an alteration in surface glycoproteins (1038). Used extensively for transport studies (247a, 1150 [review], 1152), also for studies of the mechanism of intralocus recombination (1021). Resistance is recessive in duplications from T(S1229) (PB). Recessive resistance used in a heterokaryon test system for mutation studies (1020). Suppressors obtained and used for selecting other resistance mutants (106, 107, 555, 1018). Allele 26 is a putative frameshift mutation reverted by ICR170 (106, 107). mtrascospores are slow to darken and mature; up to 50% of the young ascospores from heterozygous crosses are white (152, PB). With probable allele MN18, ascospore viability is improved by the addition of peptone to the crossing medium when the male parent is added (152). mtr has been scored on media containing 10 or 70 µg of filter-sterilized 4-methyltryptophan per ml or on 20 or 60 µg of p-fluorophenylalanine per ml (550, 1021, PB). Unlike 4-methyltryptophan, p-fluorophenylalanine is heat stable and can be added before autoclaving. Strains with mutations at the mtr locus may be obtained by selection for resistance to numerous agents or for defects in uptake ability. Thus, there is confusion in nomenclature. Genes originally designated neua, neur, neut, tru(628) may be mtr alleles. mtr was initially called mt (602).	IVR	B
pmg	IIL. Linked to pyr-4. Right of ro-3 (4%) (29%). Centromere linked. Not linked to mtr(pmn) or to pmb in IVR (248). (Previous report of mating type linkage [862] not confirmed). Greatly reduced in general amino acid transport system II (as defined in reference 777). Reduced uptake of arginine and phenylalanine. Selected by resistance to p-fluorophenylalanine in a neutral (system I), basic (system III) double mutant on medium lacking ammonium ions, where system II would be derepressed in the wild type (248, 862, 863). A non-metabolizable substrate specific for this transport system has been found (767). Called pm g. See Transport.	IIL	B