FGSC #2576

Reporting Genes: cyh-1

Species: crassa

Allele: 54(r)

Mutagen: UV

Depositor: WK

Linkage Group: IR

Mating Type: A

Species Number: 10

Genetic Background: SL

ref1: Neuhauser et al Molec Gen Genet 106:180 1970, https://doi.org/10.1007/bf00323837

ref2: Pongratz & Klingmuller Mol Gen Genet 124:35g 1973, https://doi.org/10.1007/bf00267664

Genes

Locus	Cultural Requirements	Link Group	Type
cyh-1	IR. Right of nit-1 (6%). Left of T(STL76) and al-2 (8 to 13%) (496, 797, 808).Resistant to cycloheximide (496, 748). Resistance is recessive in duplications (1090). Dominance reported in forced heterokaryons (496, 748) may have been due to skewed nuclear ratios (1090). Protein synthesis on ribosomes of the mutant cyh-1 proceeds in the presence of cycloheximide in a cell-free system (834). Readily scored on slants with 10 µg of cycloheximide per ml autoclaved in the medium. Excellent as a marker and valuable for selecting somatic recombinants or deletions in heterozygous duplications (748, 1091). Used to show that the cycloheximide-induced phase shift of the circadian clock involves protein synthesis (738). Called act-1: actidione resistant-1.	IR	B

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