FGSC #2549

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Reporting Genes: Has been replaced by strain number: 3310

Species: crassa

Allele: PBJ1;70007c

Mutagen: UV

Depositor: TEJ

Linkage Group: VR;IVR

Mating Type: a

Species Number: 10

Genetic Background: M

ref1: https://doi.org/10.4148/1941-4765.1798

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
col-1	IVR. Linked to pan-1 (0/47 asci) and cot-1 (3%) (46, 374). Colonial morphology, with no macroconidiation. Growth cyclic at moderate temperatures; steady at 38°C. Double mutant col-1 cot-1 grows better than col-1 at 24°C and better than cot-1 at 38°C (374). The double mutant pe;col-1forms microconidia (46) and was used in early mutation studies, but col-4;pe fl was found to be better (386). It was found (416) that pe;col-1 strains form microconidia at 25°C but form macroconidia at 35°C. Cell wall analysis; photograph (278). col-5 and col-8 not tested for allelism.	IVR	B
per-1	VR. Right of asp (26%) and at (8 to 14%). Left of ilv(4%) (489, PB) and ts(25%) (527). Perithecial walls are devoid of black pigment when the female parent carries per-1, regardless of genotype of the fertilizing parent (489, 490, 527). Alleles are of two types (490). Type I produces young, completely white perithecia that become pale yellowish after several days, and per-1 ascospores are white (e.g., alleles PBJ1, ABT8, and AR174). Type II produces mature perithecia that are somewhat darker orange with black pigment in the neck, and per-1ascospores are normal black (e.g., alleles 29278, 29-281, and UG1837). Unlike the perithecial wall trait, the ascospore trait shows no maternal effect. Black pigment develops in a ring around the ostiole of type II perithecia, but is pale or lacking in type I perithecia (490). Mosaic perithecia from heterokaryons have been used for a clonal analysis of perithecial development (527, 528). Expression is completely autonomous in ascospores (photographs in reference 529) and at least partially so in the perithecial walls (527-529). Used to test for variegated-type position effect, with negative results (532). White per-1ascospores (type I) germinate without heat shock and are usually killed by hypochlorite or by the 30-min, 60°C treatment used to activate normal ascospores (490, 527). Beaks of perithecia homozygous for allele PBJL (type I) are abnormal, and ascospores are not shot properly (N.B. Raju, personal communication). Type I alleles initially called sw: snow white (527).	VR	B