FGSC #2512

Reporting Genes: kyn-1;leu

Species: crassa

Allele: RC71-3;no#

Alternate Strain Number: RC71-3a

Mutagen: NG

Depositor: PJR

Linkage Group: VII;--

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Schlitt et al J Bact 117:1117-1120 1974, https://doi.org/10.1128/jb.117.3.1117-1120.1974

Genes

Locus	Cultural Requirements	Link Group	Type
leu	For biosynthetic pathway, see Fig. 15. Leucine mutants have been used extensively for studies of regulation (see references 427 and 833). Leucine mutants acquire suppressors when grown on Difco agar-sorbose medium. The suppressors are leaky auxotrophs blocked at various steps in sulfur metabolism; apparently these blocks allow more efficient use of the traces of leucine in the agar (425; S. R. Gross, personal communication). Most aliphatic and aromatic amino acids can inhibit growth of leucine mutants at appropriate concentrations, probably because of competition for a common uptake system (S. R. Gross, personal communication). For regulation, see individual loci; reviewed in references 427 and 642.		B
kyn-1	VII. Linked to nic-3 (30%) and wc-1 (20%) (G. Lester and P.J. Russell, personal communication).Partially defective in induction of the kynurenase I (inducible) isozyme by kynurenine, indole, or tryptophan, but has normal levels of the constitutive kynureninase II isozyme. Possibly regulatory. Scored and selected by a low level of anthranilate accumulation on medium supplemented with a high level of tryptophan, which results in a low level of UV fluorescence compared with that of the wild type (926).	VII	B

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