FGSC #2488

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Reporting Genes: ssu-8 ad-3B al-2;pan-2

Species: crassa

Allele: Y319-37;12-21-76 Y112M38 Y153M96

Alternate Strain Number: 76-1749-11-3

Mutagen: HA

Depositor: AJG

Linkage Group: IR IR IR;VIR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Newcombe & Griffiths 1973 Genetics 75:615-622, https://www.genetics.org/content/genetics/75/4/615.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ssu-8	IR. Linked to al-2 (2 to 8%) (145). Allele Y319-37 selected as suppressor of an aro(p) nonsense allele (145). Also suppresses certain nonsense alleles of trp-3, his-3 (reference 145) and ad-3B (reference 749). Allele Y319-37 (FGSC no. 1749), also called 54-su37, was initially thought to be a possible allele of ssu-2 and thus was listed as ssu-2 (?); later it was designated ssu-8 (145). It has been erroneously called ssu-7 in various FGSC lists and by Griffiths (408) and has been erroneously stated to be in linkage group VI (the location of the real ssu-7).	IR	B
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
al-2	IR. Right of os-5 (<1%) and T(STL76). Left of arg-6(1%) and al-1 (797, 802, 808, 816, 818). Included in duplications from Tp(T54M94), confirming location left of arg-6(808). (482) Carotenoids absent or abnormal, but steroids produced (398). Blocked in microsomal fraction and defective in phytoene synthetase (445), a particulate enzyme (445 and references cited therein) (Fig. 9). Tracer experiments indicate a lesion between prephytoene pyrophosphate and phytoene (572). Alleles include those resulting in white and pale rose-white, e.g., 15300 and Y254MI65 (1042), and purple, e.g., MN58a (154). For complementation, see references 500 and 1041. Fine-structure mapping (500, 1042) needs reevaluation because of new information on the location of the arg-6 marker (797).	IR	B
pan-2	VIR. Right of rib-1 (<1 to 3%). Left of del (6%) and trp-2(11%) (140, 141, 143, 818, PB). Unable to convert ketovaline to ketopantoic acid (138, 140, 141). Used in major studies of intralocus recombination and complementation (140-143). pan-2ascospores remain white or pale if the crossing medium is not supplemented, even when the protoperithecial parent is pan-2+. Asci in which gene conversion has occurred at pan-2 can thus be recognized and isolated (1072, 1073); photographs (1072). For good recovery of pan-2progeny, crossing media should be supplemented with pantothenic acid (10 µg/ml) even when the protoperithecial parent is pan+. Called group B.	VIR	B