FGSC #2483

Reporting Genes: ad-3B;ssu-5

Species: crassa

Allele: 2-17-34;Y319-45

Alternate Strain Number: 34-1751-D5

Depositor: AJG

Linkage Group: IR;--

Mating Type: A

Species Number: 10

ref1: Newcombe & Griffiths 1973 Genetics 75:615-622, https://www.genetics.org/content/genetics/75/4/615.full.pdf

Genes

Locus	Cultural Requirements	Link Group	Type
ad-3B	IR. Between ad-3A (0.1 to 0.7%) and nic-2 (3%) (271). (482) Uses adenine or hypoxanthine (682). Blocked in interconversion of AIR to CAIR (348) (Fig. 8). Produces purple pigment, permitting direct visual selection (276, 682). Pigment is secreted with low concentrations of adenine (e.g., 0.1 mM), not with high concentrations (2 mM) (276, 682, 785). Pigment production used to assess effect of histidine and tryptophan on purine nucleotide synthesis (786). Reduced interallelic fertility (264, 407). Complementation maps (268, 274). Relation of mutagens to complementation patterns (269). Mutants with non-polarized complementation patterns on the right side of the complementation map grow on minimal medium if supplied with CO2; other mutants do not respond to CO2, (270). Used extensively for mutagenesis (see ad-3A). Rearrangement T(I- >III)Y112M4i ad-3B, which has a breakpoint inseparable from ad-3B, was the first insertional translocation to be reported for fungi (266). Allele 7-017-0137 shows "fixed instability," mutating to an unstable prototrophic allele (41). Alleles 2-17-126, 12-21-28, and numerous others are supersuppressible (408, 749, 955). Called complementation group B.	IR	B
ssu-5	III or IV (145). Allele Y319-45 selected as a suppressor of a nonsense allele of aro(p) (145). Also suppresses the nonsense mutation trp-3 (td140) and certain ad-3Balleles, but not am(17) (see references 145, 749, and 957).	III or IV	B

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