FGSC #2377

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Reporting Genes: sor-3 ad-4;sor-1 ylo-1

Species: crassa

Allele: 17(r) K5-M27;10(r);Y30539y

Depositor: WK

Linkage Group: III IIIR;VIL VIL

Mating Type: A

Species Number: 10

ref1: Klingmuller 1967 Molec Gen Genet 100:109-116 XK56

ref2: 100:256-263 XK56

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
ad-4	IIIR. Right of met-8 (1 to 4%) and com (0 to 5%). Left of leu-1 (1 to 3%) (219, 815). (482) Requires adenine. Cannot use hypoxanthine or inosine (661). Growth on adenine (at least for strains carrying allele 44206) is improved by the addition of histidine and still more by histidine plus methionine (661). Structural gene for adenylosuccinase, which controls two reactions in adenine synthesis (393, 1158). (See Fig. 8.) Accumulates a small amount of purple pigment when adenine is limiting (682). Used for the first demonstration of complementation between alleles in vivo (393) (simultaneous with independent demonstration in am) and in vitro (1157). Enzyme in revertants (1158). Used to study purine transport (787). Strains carrying alleles 44206 and 44415 are heat sensitive (34 C versus 25 C) (482, 682) and are osmotic remediable at 30°C (636); enzyme synthesized at 30°C by heat-sensitive strains has altered properties (636). Called complementation group F.	IIIR	B
sor-1	VIL. Left of ylo-1 (3%) (560). Defective in sorbose uptake (561) and thereby resistant to growth restriction by sorbose (560). Recessive (562, 564). Symbol changed from sor-A (853).	VIL	B
sor-3	IIIR. Linked to ad-4 (7%) (560). Resistant to growth restriction by sorbose (560). Recessive with respect to colony size. Partially recessive for percent conidial germination on sorbose test media (562, 564). In heterokaryons with sor-1 or sor-2, the phenotype is intermediate between the resistant single mutant phenotype and the sensitive wild type (562). Symbol changed from sor-C (853). Called sorr-17(560).	IIIR	B
ylo-1	VIL. Between cys-1 (8%) and ad-1 (6%). Probably right of Bml (2%) (1012, PB). (381). Yellow carotenoids (381). Affects synthesis of neurosporaxanthin (4'-apo-beta'-caroten-4'-oic acid); citations in reference 398. Lesion probably involves the conversion of lycopene to 3,4-dehydrolycopene or the conversion of either torulene or gamma-carotene to neurosporaxanthin (398 and references therein) (Fig. 9). Resembles the orange wild type in young cultures, but color differences become clear with age. Expressed in both conidia and mycelia. Undefined modifiers affect intensity. Fails to complement with many of the al-1 and al-2 albino strains (R.E. Subden, personal communication).	VIL	B