FGSC #2320

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Reporting Genes: hlp-1;ff-1;his-3

Species: crassa

Allele: B538;T30;K458

Alternate Strain Number: H183

Mutagen: UV

Depositor: HCC

Linkage Group: VIIR;IIR;IR

Mating Type: A

Species Number: 10

Genetic Background: M

ref1: Ho 1969. Genetics 62:725-733 XH48, https://www.genetics.org/content/genetics/62/4/725.full.pdf

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
hlp-1	VIIR. Between sfo (1 to 9%) and nt (28 to 37%). Left of hlp-2 (8 to 25%) (458).Enables strains carrying a his-3 allele to use L-histidinol. This is proposed to be due to increased uptake through basic L-amino acid transport (system III as defined in reference 778). The hlp-1 mutation confers increased sensitivity of lys and argmutants to inhibition by arginine and lysine, respectively. (458)	VIIR	B
ff-1	IIR. Between aro-1 (5%) and un-20 (4%) (1052). (1053)Female sterile with no protoperithecia (1052, 1053). Enhanced glycerol utilization; aconidial on liquid glycerol medium. Good conidiation, but reduced aerial hyphae and heavy surface growth on sucrose minimal slants. Growth on glycerol reduces levels of both pyruvate dehydrogenase and dihydrolipoyl transacetylase (211). Effect of carbon source studied (1078). Conveniently scored by failure to produce protoperithecia on small slants of synthetic cross medium (7 days, 25 C). Allele T30 was originally recognized as specifying female sterility and was called ff-1 (1052, 1053). Allele JC744 was first characterized by glycerol utilization and called glp-3 (211).	IIR	B
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B