FGSC #2319

Reporting Genes: hlp-2;his-3

Species: crassa

Allele: HC114; 458

Alternate Strain Number: H210

Mutagen: UV

Depositor: HCC

Linkage Group: VIIR;IR

Mating Type: a

Species Number: 10

Genetic Background: M

ref1: Ho 1969. Genetics 62:725-733 XH48, https://www.genetics.org/content/genetics/62/4/725.full.pdf

Genes

Locus	Cultural Requirements	Link Group	Type
his-3	IR. Right of met-10 (R.L. Metzenberg, personal communication). Left of cog (1 to 3%) (172,174), ure-4 (1%) (78), and ad-3A (1%) (271). (434)Requires histidine (434). Complex gene coding for histidinol dehydrogenase, phosphoribosyladenosine 5'-triphosphate-pyrophosphohydrolase, and phosphoribosyl-adenosine 5'-monophosphate-cyclohydrolase (16, 673) (Fig. 14). All three activities appear to be catalyzed by a single protein (673). Strains carrying different individual alleles may lack only the early reaction(s) or only histidinol dehydrogenase, or both. Those that lack only histidinol dehydrogenase accumulate histidinol (16, 162, 1123). Mutants produce cross-reacting material (220). Used to study intralocus complementation and recombination (15, 16, 27, 162, 164, 171, 172, 1121, 1122, 1124). Intralocus recombination is regulated by cog and by rec-2 (27, 171); it is not affected by rec-1 (172). Translocation T(IR;VII)TM429, with one breakpoint in his-3, has been used to show that cog is cis-acting (171). Initial alleles: C140 and T1710 (= C1710).	IR	B
hlp-2	VIIR. Between sfo (3 to 7%) and nt (29%). Right of hlp-2(8 to 25%) (458).Enables a his-3 mutant to grow on L-histidinol. Growth of the double mutant his-3;hlp-2on histidine is inhibited by methionine, isoleucine, valine, and asparagine (458).	VIIR	B

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