FGSC #2292

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Reporting Genes: cys-3

Species: crassa

Allele: NM27(t)

Alternate Strain Number: 2138

Mutagen: UV

Depositor: DDP

Linkage Group: IIL

Mating Type: A

Species Number: 10

Genetic Background: M

Opposite Mating Type: 1272

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
cys-3	IIL. Right of pi (4%). Left of T(AR18) and pyr-4 (18 to 21%) (721, 808, 816).Uses sulfite, cysteine, or methionine; little or no response to thiosulfate (640, 721). Regulator of genes of sulfur uptake and metabolism (e.g., sulfate permease, aryl sulfatase, choline sulfatase) (284, 640, 667). Grows well on methionine. Resistant to chromate (640). Used extensively for studying regulation; for a review, see reference 642. cys-3 ascospores darken slowly or not at all, even when a cys-3 strain is the fertilizing parent and when a strain carrying heat-sensitive allele NM27t is crossed at 25°C, the permissive temperature for growth. Adding methionine to crossing medium promotes darkening but fails to give good recovery of cys-3 progeny. Recovery of a few percent cys-3 progeny is possible in well-aged crosses. (721, PB). cys-3 can be used effectively as an autonomous ascospore color mutant for demonstrating segregation patterns in asci (811; see reference 858 for photograph; see Ascospore color mutants).	IIL	B