FGSC #2256

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Reporting Genes: exo-1

Species: crassa

Allele: SF26

Alternate Strain Number: SF26

Depositor: HGG

Linkage Group: I

Mating Type: a

Species Number: 10

Genetic Background: SL

ref1: Gratzner & Scheehan J Bacteriol 97:544 1969 XG42, https://doi.org/10.1128/jb.97.2.544-549.1969

ref2: Gratzner J Bacteriol. 111:443 1972, https://doi.org/10.1128/jb.111.2.443-446.1972

Deposit Sheet ↗

Genes

Locus	Cultural Requirements	Link Group	Type
exo-1	I. Linked to mt (7%) and ad-3A (22%). Stated left of mt, but data not given (325).Hyperproduction of beta-amylase, alpha-amylase, glycoamylase, invertase (beta-fructofuranosidase), and (to a lesser extent) trehalase (404, 405, 1027). Enzymes secreted abundantly on depletion of exogenous carbon source (404). A polysaccharide is also released (325). Sevenfold increase in conidial enzyme levels. Altered amino sugar content of cell wall (404, 405). Initial allele called SF26, exoa-1 (325, 404). Probable second allele found in a strain of inl (89601) a (194, 325, 1027); allelism evidence in reference 325. With SF26, high amylase and high invertase levels cosegregated in 91 isolates (405). With allele from the 89601 strain, in a mixed-background cross, high amylase and high invertase each act as if due to a single major gene with many modifiers; high amylase and high invertase usually cosegregate and are not correlated with alkaline phosphatase levels (1027). The relation of exo-1 to gene VI-178, which reverses repression of invertase and trehalase production by mannose, is not known (663). For a linked gene defective in glycoamylase, see sor(T9) (called gla in reference 50 and called amy in reference 325). exo-1 has not been tested for allelism with sor(T9), but has been stated to be on the opposite side of mt. Because inl(89601) a has been used to obtain mutants by inositol-less death enrichment, exo-1 may be present but unrecognized in many laboratory stocks.	I	B